Place the Following Steps of Cloning in the Correct Order.

Traditional digest and ligate methods limit scientists to a single insert per cloning cycle. Checking the correct primer_bind boundaries are used.

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This way the fan leaves will still remain and speed up the.

. This method is used when there may not be restriction sites for the same enzymes in the correct place in both the vector and insert. Each part has a dropdown menu accessible via an inverted triangle. The stem of the cut branch will absorb some water while soaking.

Drag and drop to place the GFP1 and GFP2 Tags back in the correct order. Place the new cutting in the small bucket of cold water you just prepared. PCR based cloning is incredibly versatile and allows for nearly any piece of DNA to be placed into a backbone vector of choice with minimal limitations.

Background In its simplest form PCR based cloning is about making a copy of a piece of DNA and at the same time adding restriction sites to the ends of that piece of DNA so that it can be easily cloned into a plasmid of interest. This prevents air bubbles from getting trapped in the stem. After PCR this creates a copy of the target DNA region with restriction sites needed at the end of the fragment.

This menu can be used to check and if required change whether existing primer_bind annotations are used as boundaries. If your cutting has large fan leaves you should cut the blades of the leaves shorter to fit in the cloning tray.

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